Microarray Hybridization (2001)

Two groups simultaneously described the. One called it TraSH (transposon-site hybridization); the other didn’t name it.

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Badarinarayana V, Estep PW 3rd, Shendure J, Edwards J, Tavazoie S, Lam F, Church GM. Selection analyses of insertional mutants using subgenic-resolution arrays. Nat Biotechnol. 2001 Nov;19(11):1060-5. PubMed PMID: 11689852

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Sassetti CM, Boyd DH, Rubin EJ. Comprehensive identification of conditionally essential genes in mycobacteria. Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12712-7. Epub 2001 Oct 16. PubMed PMID: 11606763; PubMed Central PMCID: PMC60119.

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The microarray-based detection enabled quantitative analysis of mutant populations. The scalability of the approach enabled suppressor screens in which mutagenesis in specific mutant backgrounds revealed entire networks of regulators

Detailed methods have been published for conducting the TraSH protocol. Furthermore, additional variations were developed later.

Limitations of the technique included designing and printing the microarray for each species/strain to be examined. Additionally, technical issues in microarray hybridization can present challenges.

To combat these challenges…

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Next: Transposon Sequencing (2009)