=================================================== History and Overview =================================================== Transposon sequencing is multiplex forward genetic analysis of bacterial genes in a competitive context. The key requirement for a successful transposon sequencing study is opportunity for growth of the mutant library. Using the technique `Genetic Footprinting `__, it was shown that 15 generations is sufficient for mutants with a severe fitness defect to be effectively eliminated from detection: .. figure:: images/hare_2001_fig3.jpg :scale: 30 % :align: center :alt: Hare 2001 Important to note that transposon sequencing defines genes/operons required under a given condition, not necessarily genes that are expressed at a high level under that condition. `These groups often do not overlap `__. This is worth thinking about in the context of the question you seek to answer. .. toctree:: history_stm history_microarray history_tnseq variations