2018 Transposon Insertion Sequencing Workshop

Instructor: Mark Mandel

Dates: August 13-14, 2018

Day 1: Morning (9:30 am - 12:30 pm)

Note: Part of Jetstream that we will use will be down starting at 10:00 am on Tuesday. Therefore, we will start right in on the hands-on portion, and tomorrow we will discuss more about the history & implementation of INSeq.

Command line intro/refresher. Cheat sheet

Log into the cloud (XSEDE Jetstream)

Day 1: Afternoon (1:30 pm - 4:30 pm or later)

Install the pyinseq pipeline

Run the data analysis pipeline

Download your results

Review results

Day 2: Morning (9:30 am - 12:30 pm)

Historical context for INSeq

Considerations for experimental design

Transposon selection and design

Variations on the theme: alternate options to consider

Considerations for data deposition

Resources

Workshop docs rendered

Workshop docs source